The MR test medium contains peptone, glucose and a phosphate buffer (Stout et al, 45). To perform the MR test, I used the stabbing technique to inoculate the MR media. I sterilized the stabbing tool under an open flame, got a small amount of unknown bacteria, and stabbed the MR holder. Once the MR medium was inoculated, I let it incubate for 24 hours in the warm room at 37°C. After the 24-hour incubation, I added 15 drops of methyl red to the MR media to test for mixed acid production. The VP test (also known as the butanediol fermentation test) contains peptone, glucose and a phosphate buffer in its media, which is the same media as the MR test (Stout et al, 47). To perform the VP test, I used the injection technique to inoculate the VP media. I sterilized the stabbing tool under an open flame, got a small amount of unknown bacteria, and stabbed the VP holder. Once the VP medium was inoculated, I let it incubate for 24 hours in the warm room at 37°C. After the 24 hour incubation, I added 15 drops of Barrits Reagent A and Barrits Reagent B to the VP medium, for a total of 30 drops. Citrate medium, also known as Simmons Citrate Agar, contains sodium citrate as the sole carbon source and ammonium phosphate as the sole nitrogen source (Stout et al, 43). To perform the citrate test, I used the injection technique to inoculate the media with citrate. I sterilized the cutting tool under an open flame, got a small amount of unknown bacteria and stabbed the citrate medium. Once the Citrate medium was inoculated, I let it incubate for 24 hours in the warm room at 37°C. After the 24 hour incubation, I tested the citrate media for a change in the pH of the media, indicated by a color change to blue or green. The Urease test has a liquid... in the center of the paper. .....gelatin test result. The only test that did not correspond to the identification of E. aerogenes was the VP test. Possible errors made to not obtain an accurate test could be not waiting a full hour to see the color change or even not using the correct reagent.TSIA H2S Indole Motility MR VP Citrate Urease GelatinA/A +g (-) ( -) (+ ) (-) *(-) (+) (-) (-)It is important to know the identification of the bacterium E. aerogenes because it is pathogenic and is known to cause infections. References: Leboffe, Michael J. and Burton E. Pierce. Microbiology: Laboratory Theory and Application.Englewood, CO: Morton Pub., 2010. Print.Madigan, Michael T. et al. Brock biology of microorganisms. 13th ed. California: Benjamin Cummings, 2012. Print.Stout, M.A., et al. "Microbiology laboratory notebook". Laboratory manual. University of Texas. Arlington. 2014. Print.